Background and Objectives: Autoimmune diseases bring a heavy economic and medical burden.Epigenetics抑制剂 To systematically evaluate the effects of treatment with substances containing saponins, flavones, and alkaloids in animals with autoimmune diseases(AIDs). Methods and Study Design: The protocol for this systematic review and metaanalysis was prospectively registered with PROSPERO(CRD42023395741). Searches were conducted in the China National Knowledge Infrastructure, Wanfang, Chinese Science and Technology Journals, China Biomedical, PubMed, Cochrane Library, and Embase databases to screen for animal studies investigating the therapeutic effects of saponins, flavones, or alkaloids on autoimmune diseases; consequently, corresponding data extraction tables were prepared. SystemaDS-3201价格tic Review Centre for Laboratory Animal Experimentation was used to assess the risk of methodological bias in the included literature. RevMan 5.4 was used for the meta-analysis on the 8 serum cytokines. Results: A total of 31 studies were included, all of which were randomized consolitary intrahepatic recurrencetrolled studies. Meta-analysis indicated that substances rich in saponins, flavones, and alkaloids reduced serum levels of interleukin-1β(SMD =-1.94, 95% CI [-2.99,-0.90], P = 0.0003), interleukin-6(SMD =-1.65, 95% CI [-2.33,-0.97], P < 0.00001), interleukin-17(SMD =-2.41, 95% CI [-3.61,-1.20], P < 0.0001), tumor necrosis factor α(SMD =-1.84, 95% CI [-2.61,-1.06], P < 0.0001), and interferon γ(SMD =-1.54, 95% CI [-2.43,-0.65], P = 0.0007), but increased serum levels of interleukin-4(SMD = 1.30, 95% CI [0.15, 2.44], P = 0.03) and interleukin-10(SMD = 2.05, 95% CI [1.39, 2.70], P < 0.00001) in animal models. However, no significant regulatory effect of these three active components was observed on serum levels of interleukin-2(SMD =-0.63, 95% CI [-1.82, 0.57], P = 0.30). Conclusions: Substances containing saponins, flavones, and alkaloids regulated the changes of immune-related cytokines, it may be a novel dietary substance to relieve and control autoimmune diseases in the future.